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Hill plot

Graphical method for analysing binding of a molecule A to a macromolecule P with n binding sites. A Hill plot of lg(Θ/(1-Θ)) vs lg(A) has a slope of 1 if binding is non-co-operative and >1 for co-operative binding, where Θ = (A)bound/n(P)total is the fraction of sites occupied.

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